Search results for " PTH"
showing 10 items of 17 documents
Mid-region parathyroid hormone-related protein (PTHrP) binds chromatin of MDA-MB231 breast cancer cells and isolated oligonucleotides “in vitro”
2006
We have previously shown that PTHrP(38-94)-amide restrains growth and invasion "in vitro", causes striking toxicity and accelerates death of some breast cancer cell lines, the most responsive being MDA-MB231 whose tumorigenesis was also attenuated "in vivo". PTHrP(38-94)-amide contains the domain implicated in the nuclear import of PTHrP. Although the nucleus was identified as a destination for mid-region PTHrP, evidence for direct DNA-binding capability is lacking to date. Here, we examined the localization of PTHrP(38-94)-amide within MDA-MB231 cells and within metaphase spread preparations and characterized its DNA-binding properties, employing a combination of immunocytochemical, cytoge…
Midregion PTHrP and Human Breast Cancer Cells
2010
PTHrP is a polyhormone undergoing proteolytic processing into smaller bioactive forms, comprising an N-terminal peptide, which is the mediator of the “classical” PTH-like effect, as well as midregion and C-terminal peptides. The midregion PTHrP domain (38-94)-amide was found to restrain growth and invasionin vitroof some breast cancer cell lines, causing striking toxicity and accelerating death; the most responsive being MDA-MB231, whose tumorigenesis was also attenuatedin vivo. In addition, midregion PTHrP appears to be imported in the nucleoplasm of cultured MDA-MB231 cells andin vitro, it can bind chromatin of metaphase spread preparations and also an isolated 20-mer oligonucleotide, the…
Utilidad de la Paratohormona intraoperatoria en la cirugía del hiperparatiroidismo primario: importancia de su porcentaje de descenso y su valor fina…
2019
El hiperparatiroidismo primario (HPTP) es uno de los trastornos endocrinológicos más frecuentes y supone la primera causa de hipercalcemia en pacientes no hospitalizados. Su diagnóstico aumentó a partir de la década de los 70 del siglo pasado en los países industrializados tras la incorporación del calcio sérico a los análisis automatizados de cribado de rutina. Aunque es difícil establecer la prevalencia real de la enfermedad debido a la existencia de numerosos casos asintomáticos no diagnosticados, en la actualidad se estima una prevalencia del 0,2-1% de la población adulta, siendo la incidencia aproximada de unos 20-30/100.000 habitantes por año. La presentación clínica del HPTP ha evolu…
Intraoperative measurement of parathyroid hormone: A Copernican revolution in the surgical treatment of hyperparathyroidism
2016
Intraoperative parathyroid hormone (PTH) monitoring in the setting of the operating room represents a valuable example of the rationale use of the laboratory diagnostic in a patient-oriented approach. Rapid intraoperative PTH (ioPTH) assay is a valid tool for an accurate evaluation of the success of parathyroid surgery. The reliability of the user-friendly portable systems as well as the collaboration between operators and surgical staff allow the one-site monitoring of the ioPTH decrements on the course of the surgical management of hyperparathyroidism. The rapid answer provided by an effective decrement of PTH during parathyroidectomy contributes dramatically to the efficacy of parathyroi…
PTHrP [38-94]-amide is a DNA-binding factor: cytogenetic and molecular evidence and biological effect on normal and neoplastic human breast cells
2004
Il PTHrP [38-94]-amide è un fattore “DNA-binding”: dati citogenetica e molecolari ed effetto biologico su cellule epiteliali mammarie immortalizzate …
2004
Metilazione del DNA in artrite reumatoide
2005
Lo stato di metilazione del DNA genomico e del gene PTHrP è stato valutato con tecniche molecolari e citogenetiche in artrite reumatoide (AR), patologia autoimmune caratterizzata anche da alta incidenza di linfomi e da ipercalcemia per overespressione del gene PTHrP. La metilazione del DNA, infatti, ha un ruolo critico nello sviluppo delle malattie neoplastiche; il gene PTHrP avendo tre promotori uno dei quali contiene un’isola CpG è un buon candidato per la deregolazione da alterato pattern di metilazione locale. Le indagini sulla metilazione genomica, condotte su DNA estratto da sangue periferico di pazienti e di donatori e amplificato in reazioni di Methylation-Sensitive Arbitrarily Prim…
Treatment adherence and QoL with intermittent PTH therapy: Results from a multicentre Italian study after 6 months
2011
This ongoing multicentre study was carried out to investigate the adherence to intermittent parathyroid hormone treatment and the effect on QoL in a typical Italian population eligible for drug reimbursement (i.e. a new vertebral or hip fracture following at least one year of anti-resorptive therapy or three severe vertebral fractures or two severe and one femur fracture). 204 female patients (mean age: 72.6±8.3) were enrolled between March 2008 and April 2009. These patients were treated with PTH (PTH 1–34 or PTH 1–84) according to Italian legislation. Here, we report the results obtained at month 6.
Severe senile osteoporosis: synergism of action between PTH 1-34 and rehabilitation
2012
An analysis of the data shows that the PTH 1-34 in association with specific rehabilitation treatment is effective in reducing early pain borne by the dorsal-lumbar spine, to avoid the risk of new fractures, improve balance and gait to correct a good quality of life. Even taking into account the small number of patients, we emphasize the absence of new osteoporotic fractures in women who have also physical therapy and therapeutic exercise.